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Structural Studies and Characterization of Proteins by X-ray Crystallography and Nuclear Magnetic Resonance. Structural investigations and biophysics of molecular mechanisms of functional proteins.

Grant number: 15/13765-0
Support Opportunities:Regular Research Grants
Start date: November 01, 2015
End date: May 31, 2018
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Raghuvir Krishnaswamy Arni
Grantee:Raghuvir Krishnaswamy Arni
Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

This collaborative research project aims to express, characterize and determine the crystal structures of a number of enzymes. Crystallography provides accurate, static images of the three-dimensional structures of biological macromolecules at the atomic level that is unsurpassed by any other technique in its degree of detail and precision.Structural models help elucidate enzyme mechanisms and to comprehend complex biochemical processes inherent to life and biotechnology and to suggest structural modifications to inhibit the functioning of molecules that trigger or attenuate diseases and illnesses or to improve the performance of enzymes. The complimentary techniques of Nuclear Magnetic Resonance will be used to determine modes of binding and inhibition. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CORONADO, MONIKA APARECIDA; EBERLE, RAPHAEL JOSEF; BLEFFERT, NICOLE; FEUERSTEIN, SOPHIE; OLIVIER, DANILO SILVA; DE MORAES, FABIO ROGERIO; WILLBOLD, DIETER; ARNI, RAGHUVIR KRISHNASWAMY. Zika virus NS2B/NS3 proteinase: A new target for an old drug - Suramin a lead compound for NS2B/NS3 proteinase inhibition. Antiviral Research, v. 160, p. 118-125, . (16/08104-8, 15/13765-0, 15/18868-2, 09/53989-4, 16/12904-0)
CORONADO, MONIKA APARECIDA; OLIVIER, DANILO DA SILVA; EBERLE, RAPHAEL JOSEF; DO AMARAL, MARCOS SERROU; ARNI, RAGHUVIR KRISHNASWAMY. Modeling and molecular dynamics indicate that snake venom phospholipase B-like enzymes are Ntn-hydrolases. Toxicon, v. 153, p. 106-113, . (16/08104-8, 16/12904-0, 15/18868-2, 09/53989-4, 15/13765-0)
MARIUTTI, RICARDO B.; HERNANDEZ-GONZALEZ, JORGE E.; NASCIMENTO, ANDREY F. Z.; DE MORAIS, MARIANA A. B.; MURAKAMI, MARIO T.; CARARETO, CLAUDIA M. A.; ARNI, RAGHUVIR K.. A single P115Q mutation modulates specificity in the Corynebacterium pseudotuberculosis arginine repressor. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1864, n. 7, . (18/07977-3, 15/13765-0, 16/19995-0, 15/18868-2, 18/10736-8, 16/24587-9)
LOPES, TAIRINE ZARA; DE MORAES, FABIO ROGERIO; TEDESCO, ANTONIO CLAUDIO; ARNI, RAGHUVIR KRISHNASWAMY; RAHAL, PAULA; CALMON, MARILIA FREITAS. Berberine associated photodynamic therapy promotes autophagy and apoptosis via ROS generation in renal carcinoma cells (vol 123, 109794, 2020). BIOMEDICINE & PHARMACOTHERAPY, v. 125, p. 1-pg., . (14/22198-0, 15/13765-0, 15/16660-5)
BHAT, SHREESHA K.; JOSHI, MANJUNATH B.; VASISHTA, SAMPARA; JAGADALE, RAJESH N.; BILIGIRI, SETLUR G.; CORONADO, MONIKA A.; ARNI, RAGHUVIR K.; SATYAMOORTHY, KAPAETTU. P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, . (15/13765-0)
EBERLE, RAPHAEL J.; CORONADO, MONIKA A.; PEINADO, RAFAELA S.; DE MORAES, FABIO R.; OLIVIER, DANILO; DREYER, THIAGO; LOPES, DEBORA DE OLIVEIRA; ROSA DA LUZ, BRENDA SILVA; AZEVEDO, VASCO; ARNI, RAGHUVIR K.. The polyanions heparin and suramin impede binding of free adenine to a DNA glycosylase from C. pseudotuberculosis. International Journal of Biological Macromolecules, v. 125, p. 459-468, . (09/53989-4, 15/18868-2, 16/08104-8, 15/13765-0, 16/12904-0)
EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; TASIC, LJUBICA; ARNI, RAGHUVIR K.; CORONADO, MONIKA A.. Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis. International Journal of Biological Macromolecules, v. 107, n. B, p. 1999-2007, . (16/08104-8, 15/13765-0, 15/18868-2, 09/53989-4, 16/12904-0)
DA SILVA, THAIS PEREIRA; DE CASTRO, FERNANDO JACOMINI; VUITIKA, LARISSA; POLLI, NAYANNE LOUISE COSTACURTA; ANTUNES, BRUNO CESAR; BOIA-FERREIRA, MARIANNA; MINOZZO, JOAO CARLOS; MARIUTTI, RICARDO BARROS; MATSUBARA, FERNANDO HITOMI; ARNI, RAGHUVIR KRISHNASWAMY; et al. Brown Spiders' Phospholipases-D with Potential Therapeutic Applications: Functional Assessment of Mutant Isoforms. BIOMEDICINES, v. 9, n. 3, . (15/18868-2, 15/13765-0)
EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; OLIVIER, DANILO; DO AMARAL, MARCOS S.; TASIC, LJUBICA; AMI, RAGHUVIR K.; CORONADO, MONIKA A.. Inhibition of thioredoxin A1 from Corynebacterium pseudotuberculosis by polyanions and flavonoids. International Journal of Biological Macromolecules, v. 117, p. 1066-1073, . (09/53989-4, 15/18868-2, 16/08104-8, 15/13765-0, 16/12904-0)
GREMSKI, LUIZA HELENA; DA JUSTA, HANNA CAMARA; DA SILVA, THAIS PEREIRA; POLLI, NAYANNE LOUISE COSTACURTA; ANTUNES, BRUNO CESAR; MINOZZO, JOAO CARLOS; WILLE, ANA CAROLINA MARTINS; SENFF-RIBEIRO, ANDREA; ARNI, RAGHUVIR KRISHNASWAMY; VEIGA, SILVIO SANCHES. Forty Years of the Description of Brown Spider Venom Phospholipases-D. TOXINS, v. 12, n. 3, . (15/13765-0, 15/18868-2)
PEINADO, RAFAELA DOS S.; OLIVIER, DANILO S.; EBERLE, RAPHAEL J.; DE MORAES, FABIO R.; AMARAL, MARCOS S.; ARNI, RAGHUVIR K.; CORONADO, MONIKA A.. Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B-12 Metabolism. SCIENTIFIC REPORTS, v. 9, . (09/53989-4, 15/18868-2, 16/08104-8, 15/13765-0, 16/12904-0)
BHAT, SHREESHA K.; JOSHI, MANJUNATH B.; ULLAH, ANWAR; MASOOD, REHANA; BILIGIRI, SETLUR G.; ARNI, RAGHUVIR K.; SATYAMOORTHY, KAPAETTU. Serine proteinases from Bothrops snake venom activates PI3K/Akt mediated angiogenesis. Toxicon, v. 124, p. 63-72, . (15/13765-0)
EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; TASIC, LJUBICA; ARNI, RAGHUVIR K.; CORONADO, MONIKA A.. Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis. International Journal of Biological Macromolecules, v. 107, p. 9-pg., . (09/53989-4, 15/13765-0, 16/08104-8, 15/18868-2, 16/12904-0)

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